Acharya Arpita

CRISPR/Cas9 Construct Development for Knock-out of Root Architecture Associated 1 Gene in Rice (Oryza sativa L.)

Acharya Arpita, G Bhavya, Abida P.S, Kiran A.G., Preetha R., Melna Mary C.J & G.K. Krishna · Journal of Advances in Biology & Biotechnology · 2024

Deeper rooting is an important trait for sustained yield under water-limited environments, as drought exhibits serious threat to rice (Oryza sativa L.) production. To mitigate the challenge, we aimed to modify the Oryza sativa ROOT ARCHITECTURE ASSOCIATED 1 (OsRAA1) gene, one of...

Open access Research Article 10.9734/jabb/2024/v27i121767

CRISPR/Cas9 Driven Targeted Editing of Grain Number 1a gene: sgRNA Constructs Design for Yield Enhancement in Rice (Oryza sativa L.)

G Bhavya, Acharya Arpita, Abida P.S, Preetha R., Kiran A.G., Melna Mary C.J & G.K. Krishna · Journal of Advances in Biology & Biotechnology · 2024

Yield is the ultimate trait determined by various quantitative trait loci (QTLs). One such QTL, GRAIN NUMBER 1a (GN1a), encodes for an enzyme Cytokinin oxidase/ dehydrogenase (CKX), which negatively influences the yield by degrading the phytohormone Cytokinin. CRISPR/Cas9, a prec...

Open access Research Article 10.9734/jabb/2024/v27i121778

In silico Design and Validation of CRISPR/Cas9 gRNAs for Enhancing Drought Tolerance and Yield in Rice

Shafana S, Kripa Pappachan, Abida P.S, Acharya Arpita, G Bhavya & Melna Mary C.J · Journal of Advances in Biology & Biotechnology · 2024

Rice is a staple food crop globally, and its yield is significantly impacted by drought, posing a threat to food security. Several genes have been implicated in drought response and yield regulation, which can be targeted using CRISPR/Cas9 genome editing techniques to develop imp...

Open access Research Article 10.9734/jabb/2024/v27i121852