Recombinant Expression of Taq DNA Polymerase Using pBluescript SK(+) and Chromatography-free Purification: A Cost-effective Strategy for Low-resource Laboratories
Nolan J. Ramanjulu & Bibi Rafeiza Khan · Journal of Advances in Biology & Biotechnology · 2025
Taq DNA polymerase is a cornerstone of molecular biology. However, its production typically relies on proprietary vectors and affinity-based purification systems. These requirements limit access in educational and under-resourced environments. In this study, we demonstrate the fi...
Open access
Research Article
10.9734/jabb/2025/v28i72660