An Effective Cloning, Heterologous Expression and Physiological Activity in Lactococcus lactis NZ9000 of Catalase Gene from Escherichia coli DH5α
Chongbi Li, Sainan Li, Chen Li, Yansheng Liu & Gao Feiyun · Journal of Advances in Microbiology · 2017
In order to clone active Catalase gene from bacteria, we introduced a method of shotgun integrating specific screen from Escherichia coli DH 5a. Genome DNA was extracted from E. coli DH5 and partially digested with Sau3AI. Then some fragments more than 2.26kb were collected and l...
Open access
Research Article
10.9734/JAMB/2017/34608