Luís M. A. Ferreira

Expression in Escherichia coli and Purification of Microbial Enzymes for the Production of Enzymatic Analytical Kits to Measure Relevant Metabolites

Patrícia I. P. Ponte, Catarina I. P. D. Guerreiro, Vânia Fernandes, André T. Fernandes, Carlos M. G. A. Fontes, Luís M. A. Ferreira & José A. M. Prates · Journal of Applied Life Sciences International · 2014

Aim: The present study describes the procedure for the isolation, cloning, expression and purification of eight different microbial enzymes required for the assembly of a comprehensive range of enzymatic test kits. Methodology: The genes encoding the enzymes were isolated through...