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Poonam Singh Nigam

Publications (5)

Effect of Iron and Hydrogen Peroxide Supplementation on the Total Phenols Content and Cytoxicity of Honey for MCF-7 Breast Cancer Cells

Lai Yung Wong, Poonam Singh Nigam & Richard Owusu-Apenten · Journal of Advances in Biology & Biotechnology · 2018

Aims: To evaluate the effect of supplementation with iron (II/III) salts and/ or hydrogen peroxide on Manuka honey total phenols content (TPC) and the cytoxicity toward MCF-7 breast cancer cells. Study Design:  The study had an In Vitro design. Place and Duration of Study: Ulster...

Open access Research Article 10.9734/JABB/2018/42344

Determination of Iron (III) Reducing Antioxidant Capacity for Manuka Honey and Comparison with ABTS and Other Methods

Hasif Ilyasa Mohd Yusof, Richard Owusu-Apenten & Poonam Singh Nigam · Journal of Advances in Biology & Biotechnology · 2018

Aims: Applying multiple assays with trolox as the sole reference compound is a recent AOAC proposal to improve the reliability of total antioxidant capacity determinations. The aim of this study was to evaluate, iron (III) reducing antioxidant capacity (iRAC) for Manuka honey sam...

Open access Research Article 10.9734/JABB/2018/42202

Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Extract

Tsz Ching Chau, Richard Owusu-Apenten & Poonam Singh Nigam · Journal of Advances in Biology & Biotechnology · 2017

Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Extract Aims: To evaluate total phenols content (TPC), antioxidant capacity (TAC) and antibacterial activity of Manuka honey extract (MHE) and to compare such properties with those for unfractionated M...

Open access Research Article 10.9734/JABB/2017/37101

Evaluation of Manuka Honey Estrogen Activity Using the MCF-7 Cell Proliferation Assay

Kathleen Henderson, Tahrir Aldhirgham, Poonam Singh Nigam & Richard Owusu-Apenten · Journal of Advances in Biology & Biotechnology · 2016

Aims: To assess the estrogenic activity of Manuka honey using the MCF7 cell proliferation assay. Study Design:  In vitro cell based E-screen. Place and Duration of Study: Ulster University, Coleraine, UK, September 2015 to September 2016. Methodology: Manuka honey (UMF15+) was ch...

Open access Research Article 10.9734/JABB/2016/29887

Effect of pH on the Radical Quenching Capacity of Tea Infusions Using the ABTS•+ Assay

Yuk Man Chan, Nga Kwan Cheng, Poonam Singh Nigam & Richard Owusu-Apenten · Journal of Applied Life Sciences International · 2016

Aims: The aims of this study were to assess the impact of pH on the free radical quenching activity of tea infusions using a modified 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay and three antioxidant compounds as reference. Study Design: In-vitro method....

Open access Research Article 10.9734/JALSI/2016/27235