RATHISH R L

Standardisation of 16S rRNA Gene Based Nested PCR as a Sensitive Tool for Canine Leptospirosis Diagnosis

ATHIRA K S, BIPIN K C, DEEPA P M, Janus A, RATHISH R L & MUHASIN ASAF V N · Journal of Advances in Biology & Biotechnology · 2025

Aim: The present study aimed to standardize a 16S rRNA gene-based nested PCR assay for the sensitive detection of leptospirosis in dogs and to compare its diagnostic performance with PCR targeting lipL32 and microscopic agglutination test (MAT). To achieve this, a cross-sectional...