Toshiaki Nikai

Characterization of a Novel Thrombin-like Enzyme, Globlase from the Venom of Gloydius blomhoffii (Japanese Mamushi)

Yumiko Komori, Yusuke Takashima, Shoko Ono & Toshiaki Nikai · International Journal of Biochemistry Research & Review · 2019

Aims: To elucidate the coagulation mechanisms of a novel clotting factor isolated from Gloydius blomhoffii venom, its hydrolytic activity on various substrates were examined. Furthermore the primary structure was determined and compared with the other snake venom components. Meth...

Open access Research Article 10.9734/ijbcrr/2019/v26i430106

Structure-Activity Relationship of Phospholipase A2s Isolated from Vipera aspis Venom

Yumiko Komori, Yasuo Nakamura, Izumi Yunokuchi & Toshiaki Nikai · International Journal of Biochemistry Research & Review · 2012

Aims: To elucidate the structure-activity relationship of snake venom phospholipase A2, the primary structures of two phospholipase A2s from V. aspis venom were analyzed, and the three-dimensional conformation models were compared. Study Design: Cross-sectional study. Place and D...

Open access Research Article 10.9734/IJBCRR/2012/1074

Characterization of Elastolytic Protease, Asnilase from Aspergillus nidulans and Its Cytotoxicity on Human Endothelial and Epithelial Cells

Yumiko Komori, Yoshiyuki Okumura, Kazuhito Kamiya, Kenji Ogawa & Toshiaki Nikai · International Journal of Biochemistry Research & Review · 2016

Aims: To elucidate the pathogenicity of Aspergillus nidulans, elastolytic protease was isolated from the culture supernatant of the isolate, and its biological activity and cytotoxicity were examined. Methodology: A. nidulans (NBRC 4340) spores were cultured on YCB-elastin medium...

Open access Research Article 10.9734/IJBCRR/2016/26206