Yoshiko Nishimura

Establishment of Efficient System for Isolation and Manipulation of Single Protoplasts Containing Aposporous Embryo Sac Initial Cell in Guinea Grass (Panicum maximum)

Lanzhuang Chen, Yoshiko Nishimura & Chenti Xu · Biotechnology Journal International · 2014

Establishment of Efficient System for Isolation and Manipulation of Single Protoplasts Containing Aposporous Embryo Sac Initial Cell in Guinea Grass (Panicum maximum) Aims: In order to do the molecular analysis of mechanism of aposporous embryo sac initial cell (AIC) appearance,...

Open access Research Article 10.9734/BBJ/2014/12213

Attempt to Establish Direct Gene Transformation System to Seeds of Sweet Potato (Ipomoea batatas) Using Electroporation Method

Lanzhuang Chen, Shigeru Masuoka, Yoshiko Nishimura, Tetsufumi Sakai, Yasuhiro Takahata & Chengti Xu · Biotechnology Journal International · 2017

Aims: The purpose of this study is to get transformants by using the seeds of sweet potato and the instrument of NEPA21, an electroporation method. Study Design: As the first step, we used grafting method to get seeds from the combinations of Ipomoea crassicaulis cv. [Kidachiasag...

Open access Research Article 10.9734/BJI/2017/35275