Phytochemical and Antioxidant Evaluation of Moullava spicata (Dalzell) Nicolson Leaf Extract
K. Lohith, R. Vijay, K. C. Pushpalatha, Chandrashekara G. Joshi
Annual Research & Review in Biology · pp. 188–197 · Published 4 Oct 2013
10.9734/ARRB/2014/3370Abstract
Background: Moullava spicata (Dalzell) Nicolson is an endemic medicinal plant and its leaves are used to treat many diseases. Aims: The aim of the study was to evaluate the phytochemical, antifungal and antioxidant status of Moullava spicata leaf extract. Methods and Materials: The total phenolic content of leaf was measured by Folin-Ciolteau’s assay. Antioxidant activity of the plant was detected by using in-vitro 2,2- diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonicacid) (ABTS), Phosphomolybdenum reduction and Ferric reducing antioxidant power (FRAP) assay. Antifungal activity was detected by Agar cup diffusion method. Result: The phytochemical analysis showed that extracts of M. spicata contained pharmacologically important phytochemicals. Acetone extract showed higher total antioxidants activity and reducing power. However DPPH and ABTS scavenging activity were higher in methanol and acetone extracts respectively. Conclusion: Present study showed pharmacologically significant phytochemical and high antioxidant status on leaf.
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