Evaluation of Ex-vivo Anti-arthritic, Anti-inflammatory, Anti-cancerous and Thrombolytic Activities of Mussaenda roxburghii Leaf

Abstract

Aim: The current study evaluates the anti-arthritic, anti-inflammatory, anti-cancerous and thrombolytic activities of methanolic extract of Mussaenda roxburghii leaf. Place and Duration of study: This study was conducted in the Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, during the period between August 2013 and March 2014. Methodology: Bovine serum albumin was used for the evaluation of anti-arthritic potential and methanolic plant extract was compared with diclofnac sodium. The anti-inflammatory activity was assessed by hypotonicity induced membrane lysis and methanolic plant extract was also compared with diclofenac sodium. Brine shrimp lethality bioassay was used to evaluate the anti-cancerous activity of methanolic extract of Mussaenda roxburghii. Evaluation thrombolytic activity of methanolic extract of Mussaenda roxburghii was investigated by clot lysis method and methanolic extract was compared with streptokinase. Results: In the case of anti-denaturation, methanolic extract of Mussaenda roxburghii exhibited 42.15% of inhibition at 31.25 µg/ml and 80.33% of inhibition at 1000 µg/ml concentration. For anti-inflammatory effect, methanolic extract showed 53.79% and 86.93% of membrane stabilization activity at 31.25 µg/ml and 1000 µg/ml concentration respectively. The extract showed minimal anticancerous activity, lethal concentration (LC50) value of the extract was 282.03 μg/ml compared to vincristine sulphate (12.59 µg/ml). For the evaluation of the thrombolytic activity, methanolic leaf extract of Mussaenda roxburghii was treated with human blood. It showed promising activity which was about 49.09%.