A New RP-HPLC Method for Quantitative Analysis of Atorvastatin Calcium in Bulk and Pharmaceutical Dosage form by Using Design of Experiment Technique Optimization
Elsadig H. Rudwan, Amna B. W. E Mohammed, Ahmed E. M. Saeed
International Research Journal of Pure and Applied Chemistry · pp. 1–10 · Published 15 Feb 2017
10.9734/IRJPAC/2016/31250Abstract
A rapid high-performance liquid chromatography method was developed and validated for the determination of atorvastatin calcium in pharmaceutical dosage forms, and for evaluation of its stability in stress testing. Separation of atorvastatin was successfully achieved on a C-18 column utilizing -acetonitrile, Phosphate buffer pH 3.0 at the volumetric ratio of 59:41. The detection wavelength was 245 nm. The method was validated and the response was found to be linear in the drug concentration range of 6.4 µg/mL – 9.6 µg/mL. The correlation coefficient was 0.9999. The RSD values for precision as repeatability and reproducibility were 0.377 and 0.70 respectively, and the regression equation was Y= 0.9962x+0.2241. All the validation parameters were within the acceptable range. The developed method was successfully applied to estimate the amount of atorvastatin calcium in tablets.
Cited by 1
Maja Hadzieva Gigovska, Ana Petkovska, Jelena Acevska · Macedonian Journal of Chemistry and Chemical Engineering · 2018
Related research
- Exploring Margarine in Anhydrous Milk Fat by Chromatographic Tools — shares topic coverage
- Simultaneous Estimation of Diphenhydramine Hydrochloride and Naproxen Sodium in Raw and Tablet Formulation by Reverse Phase - High-Performance Liquid Chromatographic Method — shares topic coverage
- Biological Activity of Different Batches of Equine Chorionic Gonadotropin as Determined by Reversed-phase High-performance Liquid Chromatography and in vivo Assay — shares topic coverage
- Development and Validation of a Stability-indicating RP-HPLC Method for the Estimation of Eltrombopag in Bulk and Tablet Formulation — shares topic coverage
- Simultaneous Estimation of Aspirin, Atorvastatin Calcium and Clopidogrel Bisulphate in a Combined Dosage form by RP-HPLC — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
1
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.