Antifungal Activity of Buchholzia coriacea’s Methanolic Extract on Dermatophytes Isolated from Donkeys in Katsina State, Nigeria

Abstract

Dermatophytes are filamentous fungi that affect both human and animal skin, hair, and nails. There is a public health issue with it. In order to ascertain the effects of the methanolic extracts of Buccholzia coriacea on the isolates and the sensitivity level of the isolates to common antifungal drugs, this study was developed to explore the prevalence of Dermatophytes from clinical cases in donkeys. Samples were initially cultivated on Sabouraud dextrose agar, and then on Potato dextrose agar (secondary culture). Sixty (60) clinical samples were collected out of which 13(21.7%) were positive for Dermatophytes. Species identified were; T. rubrum (3), T. verrrucossum (1), T. equinum (3), T. Mentagrophytes (3) M. audounii (1), M. gypseum (2). At concentrations between 125 and 250 mg/ml, the methanolic extract of Buccholzia coriacea demonstrated antifungal effects on every isolate with values for the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The isolates' susceptibility to six popular antifungal medications was assessed. The isolates were well inhibited by Ketoconazole and terbinafine, but none of them were susceptible to amphotericin B. This study was able to show that Buccholzia coriacea's methanolic extract has antifungal properties. Additionally, two Dermatophytes species (Trichophyton and Microsporum) from Katsina state, Nigeria, were able to be isolated in this study.