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Research Article Open access CC BY 4.0

Diagnostic Modalities in Medical Mycology: Current Methods and Emerging Tools

Ene Chidiebere Brown, Akpa Winifred Chinwedu, Nweke Idakari Chinedu, Ene Ebele Chidiebere, Onoh Toochukwu Joan, Akpa Christain Obasi, Udochukwu Kingsley Nzube, Osuagwu Chioma Stella, Okparaoka Samuel Uchechukwu, Okparaoka Vivian Chisom, Onyeyili Ikemefuna Nnamdi

Journal of Advances in Microbiology · pp. 38–49 · Published 5 Sep 2025

10.9734/jamb/2025/v25i9988

Abstract

Mycology is a rapidly evolving field of science that has great potential to improve the understanding of fungi and their impact on the world. The review explores specific diagnostic methods employed in fungal diagnosis, tracing the evolution from traditional approaches to the more advanced and sophisticated techniques utilised in contemporary mycology. It emphasises the importance of integrating both conventional and innovative tools to enhance the early detection and accurate identification of fungal pathogens. Advances in sequencing technology and other molecular techniques are expanding the knowledge of fungal diversity and function. Synthetic biology is opening up new possibilities for using fungi as biotechnological tools. Many existing diagnostic methods are time-consuming, labour-intensive, and require specialised equipment and trained personnel. Therefore, there is a need for the development of diagnostic tests that are rapid, easy to use, and can be deployed in resource-limited settings. The future of medical mycology lies in integrating cutting-edge diagnostics with therapeutic innovation and a deeper understanding of host–pathogen interactions.

Advanced imaging techniques DNA sequencing fluorescence In-situ hybridisation reflectance confocal microscopy synthetic biology

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