Association of Hepatitis Virus Infections with CD4 and CD8 among Certain Group of Lymphoma Patients in Babylon Provence

Abstract

This study was planned to detect the immunological association impact of hepatitis infection in lymphoma patients, There are two branches of this study, firstly theoretical background and secondly is practical as diagnostically and immunological impact. Investigate the immunological association of Hepatitis viruses on lymphoma patients by estimation of certain, auto antibodies and specific CD markers among clinically diagnosed patients of Hodgkin's and Non Hodgkin lymphoma admitted to malignancies registry center of Babylon. At the time limited between February 2012 and February 2013. Specific diagnosis is clinically done by consultant physician and histopathology's. The sample selection was classified as the following; newly diagnosed patients (HL, n= 17), (NHL. n= 33) and (30) healthy samples were chosen as control group as well as (9) autopsy samples examined by histopathologist and confirm it as a normal section before used as healthy lymph node control for CD markers. The examination of (CD4 and CD8) used as a monitor to evaluating the lymph node environment during infection, as well as screening test for different types of hepatitis viruses for both, patients and control. To confirm the link between immune reactivity with such condition, the Antinuclear and Anti mitochondrial antibodies also done by using immunoflourescence technique, because there are a few studies dealing with such relation and to find out the accurate and early diagnostic protocol for detection of risk patients to infect with such diseases. According to this application, the clinically significant results showed that; the age groups distributed among young age (20 -29 y) for HL, while adult age at (50-59 y) for NHL. There were no differences between males and females. The frequency of hepatitis viruses revealed that HCV is more distributed at all patient groups at a higher percent (35.64%) than HBV (11.76%) and no positive result of HAV as well as no co – infetion with viral hepatitis were seen at the time of study. Tumor infiltrating lymphocytes with positive CD4, CD8 markers could play a role in immunological microenvironment of both types of lymphoma .In conclusion mentioned that the increase of positive CD8 tumor infiltrating lymphocytes, and the ratio of CD4/ CD8 equal to (1:1) refers to that the tumor infiltrating lymphocytes are T lymphocytes, often CD8 CTLs, and there is progressive maturation of autoantibodies in association with viral activity among both lymphoma types.