Comparative In vitro Cytotoxicity Evaluation of Moringa Extract, Clitoria Extract and Coccinia grandis by MTT Assay Using Various Cell Lines

Abstract

Evaluation and development of potent anti-cancer treatments have been a global interest of research due to significant rise in cancer as well as related diseases. Many herbal extracts have shown the potential to be used in the treatment of such diseases. In order to evaluate the anticancer features of Moringa oleifera (ME), Clitoria ternatea (CE), and Coccinia grandis (CCD), this study was carried out using various cell lines i.e. L-929, Vero, L-132, Raw 264.7, and Balb 3T3 with six different concentrations of each test substance by using MTT cell viability Assay. Additionally, in-vitro studies are performed using these test items and claims are being done that these test items are playing a pivotal role either treatment of Alzheimer's diseases (AD) and Parkinson's disease (PD) or decreasing the severity and incidences of such neurodegenerative diseases. Globally, an average life expectancy rises, so does the severity of these conditions as well. Hence, this study was also aimed to determine whether any supportive changes are showed on different cell lines or not.   In contrast to normal cells, the test substances revealed possible anti-cancer characteristics when exposed to carcinoma cell lines based on the data obtained in this study. The results indicated that ME, CE and CCD are effective against cancer cells; hence based on the results of these study, it could be concluded that that the data of the present study supports the interpretation that these test items have significant effects either in treatment or slowing down the clinical signs of PD and AD under the conditions and procedures followed in the present study. Based on the results of cell viability results it could also be concluded that the test items are safer to use up to the concentrations of 500 µg/mL.