Effect of Time Interval between the Semen Collection and its Dilution on Seminal Attributes during Semen Cryopreservation in Goats

Abstract

A study was conducted to evaluate the best suitable time to dilute the fresh ejaculated semen for cryopreservation in goats. 10 healthy bucks of Sirohi breed maintained at goat frozen semen station were selected for the experiment. The semen was collected from each buck in morning hours using artificial vagina. Just after collection, the semen was divided into three groups (I, II and III) and diluted as per the experimental design viz. 0, 5 and 10 minutes post collection with tris egg yolk semen extender and subject to freezing. The frozen semen was evaluated after 7 days of storage in LN2 cylinder. On evaluation a significantly (P<0.05) higher values for percent viable sperm and mitochondria activity were observed in group II compared to I & III group. Significantly (P<0.05) higher values for percent viable ROS+ ve sperm and acrosome disrupted acrosome were observed in group I followed by Group III and II. The path velocity and kinematic characters exhibited significantly (P<0.05) higher values in group II compared to other group during experiment. The interpretation of the result suggests that both early and late dilution of neat semen result in poor post thaw semen quality. The neat semen should be diluted at 5 minutes of collection to achieve best post thaw semen quality in goats.