Antimicrobial Activity of Biosynthesized Silver Nanoparticles from Mentha arvensis and Syzygium aromaticum and Their Combinations with Levofloxacin and Cefotaxime
Okey-Orji V. N, Ngwai Y. B, Nkene I. H, Asogwa, I. S, Usman, T. F, Fagbola, O. F, Akyala, I. A, Igbawua I. N, Galleh, R. P, Tama, S. C, Abdulmumin R. I, Aremu, O. K, Simon, O. A
Asian Journal of Research in Biochemistry · pp. 59–67 · Published 3 Nov 2025
10.9734/ajrb/2025/v15i6447Abstract
Aims: This study is aimed at evaluating the antimicrobial activity of biosynthesized silver-nanoparticles (AgNPs) from Mentha arvensis and Syzygium aromaticum and its combinations with cefotaxime and levofloxacin. Study Design: Cross sectional study. Place and Duration of Study: Biological Science Department of National Institute for Pharmaceutical Research and Development Idu Abuja, between January 2025 and August 2025. Methodology: The AgNPs were biosynthesized from Mentha arvensis (M. arvensis) and Syzygium aromaticum (S. aromaticum) extracts and characterized with Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy. The antimicrobial activity of AgNPs, cefotaxime and levofloxacin against the Pseudomonas aeruginosa (P. aeruginosa) isolates (B1, B2 and B3) from surgical wound were evaluated using the microbroth dilution method. The effect of combination of the AgNPs and cefotaxime or levofloxacin was evaluated using the checkerboard method. Results: The size of S. aromaticum and M. arvensis were 50-179nm with an average of 114.5nm and 80-268nm with an average of 174nm respectively. The AgNPs biosynthesized from S. aromaticum and M. arvensis had activity against the isolates with minimum inhibitory concentrations (MICs) ranges from 6.25-0.0078 mg/mL while for cefotaxime and levofloxacin, the MICs ranges from 64.0-2.0 µg/mL. The combination of S. aromaticum/cefotaxime, S. aromaticum/levofloxacin and M. arvensis/levofloxacin had synergistic effect against only B1 with FICs ranges from 0.125-0.5 but had indifference effect on isolate B2 and B3 with FICs of 2.0. Finally, the combination of M. arvensis/cefotaxime had additive effect on B1 and B2 with FICs of 1.0. Conclusion: The biosynthesized AgNPs from S. aromaticum and M. arvensis had antipseudomonal activity with inconsistent effect in its combination with cefotaxime and levofloxacin against the P. aeruginosa isolates.
Cited by 0
No indexed citations yet.
Related research
- Antimicrobial Activity of Pyocyanin for Inhibition of Pseudomonas aeruginosa Urinary Tract Pathogens — shares topic coverage
- Dew Formation and Anaerobiosis in Anaerobic Jars — shares topic coverage
- Carbapenemase-Encoding Genes in Pseudomonas aeruginosa: Multiplex PCR Detection, Microarray Based Detection and Gene Sequencing — shares topic coverage
- Pseudomonas aeruginosa Infection in Humans and Antibiotic Resistance — shares topic coverage
- Examination of the Production of Biofilm and Acyl Homoserine Lactone (AHL) in Pseudomonas aeruginosa Isolates Exposed to Sub-inhibitory Antibiotics Concentrations — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
0
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.