The Effectiveness of Recovery of Gram-positive and Gram-negative Bacteria on Sano-Gam Media

Abstract

The present study was carried out to determine the recovery response of cold-shocked Gram-positive and Gram-negative bacteria on Sano-Gam media. Sano-Gam medium is an abbreviation of the two scientists names Sanousi and Gamer Aldawla. This medium consisted of; Sodium pyruvate, Mg, Cu, Fe and Zn, and cellular catalase enzyme. The catalase enzyme was extracted from fresh broth culture of E. coli bacterium and was incorporated in the media separately or in variety of combinations with sodium pyruvate and trace elements. The studied bacteria were Streptococcus spp., Salmonella spp., Proteus vulgaris and Bacillus cereus. All tested bacteria were obtained from one standard culture collection (Department of Microbiology, Veterinary Medicine, University of Khartoum). On Sano-Gam media the response of all tested bacteria to recovery were monitored and determined through sequential colony counting. Both Gram-positive and Gram-negative bacteria were found to have an excellent ability to repair their cellular damages and well recovered on Sano-Gam media, notwithstanding of their distinctive cell wall components.