Adsorption and Stabilization of a Raw Starch Digesting Amylase on Micro Bead Silica Gel 300 A
Tochukwu Nwamaka Nwagu, Hideki Aoyagi, Bartholomew N. Okolo
Biotechnology Journal International · pp. 85–101 · Published 14 Jun 2012
10.9734/BBJ/2012/894Abstract
Aims: To produce a robust starch hydrolyzing enzyme (improved catalytic and non-catalytic properties) by the adsorption of the soluble enzyme on micro bead silica gel. Place and Duration of Study: Department of Life Sciences and Bioengineering, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba-shi Ibaraki-ken, Japan between July 2009 and August 2010. Methodology: Ten types of Micro Bead silica gel with pore sizes ranging from 0.4-100 nm were screened to determine the best support for the immobilization of a microbial raw starch digesting amylase (RSDA). The micro bead which gave the highest yield was selected for further studies. Properties of the immobilized enzyme were compared to the free type to determine the effect of immobilization on catalytic, storage and operational stability. Results: Micro Bead 300 A gave the highest yield and the optimum condition for adsorption of the RSDA was at pH 5, 25°C for 24 h. Optimum pH of the immobilized enzyme shifted from 5 to 4.5 and optimum temperature from 30 to 50°C. The immobilized amylase retained over 70% of its initial activity after 12 h incubation at 70°C in 0.2 M citrate phosphate buffer pH 5 whereas free enzyme lost 92% initial activity under same conditions. Immobilized enzyme retained 95% activity after 10 batch reactions of 30 min each and 100% activity after storage for 6 weeks at room temperature. Conclusion: Immobilized RSDA was marginally more pH and temperature stable compared to the native type. It also exhibited storage stability and could be re-used repeatedly without considerable desorption during washing. The kinetic and stability features combined with the properties of the support make this process appealing for industrial application.
References (47)
- 1 A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding [DOI]
- 2 Improvement of enzyme activity, stability and selectivity via immobilization techniques [DOI]
- 3 Enzyme Immobilization: The Quest for Optimum Performance [DOI]
- 4 Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation [DOI]
- 5 Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance [DOI]
- 6 Microbial Enzymes and Biotechnology [DOI]
- 7 Bioinspired enzyme encapsulation for biocatalysis [DOI]
- 8 Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization [DOI]
- 9 A new, mild cross‐linking methodology to prepare cross‐linked enzyme aggregates [DOI]
- 10 A novel raw starch digesting thermostable α-amylase from Bacillus sp. I-3 and its use in the direct hydrolysis of raw potato starch [DOI]
- 11 A novel raw starch digesting α-amylase from a newly isolated Bacillus sp. YX-1: Purification and characterization [DOI]
- 12 α-Amylase immobilization on functionalized glass beads by covalent attachment [DOI]
- 13 Enhanced activity and stability of α-amylase immobilized on alumina [DOI]
- 14 Comparison of degradation abilities of α- and β-amylases on raw starch granules [DOI]
- 15 Covalent immobilisation of invertase onto a reactive film composed of 2-hydroxyethyl methacrylate and glycidyl methacrylate: properties and application in a continuous flow system [DOI]
- 16 The immobilization of a thermophilic β-galactosidase on Sepabeads supports decreases product inhibition [DOI]
- 17 Esterification in organic solvents by lipase immobilized in polymer of PVA–alginate–boric acid [DOI]
- 18 Production and properties of the highly efficient raw starch digesting α-amylase from a Bacillus licheniformis ATCC 9945a [DOI]
- 19 Immobilization of alpha-amylase produced by Bacillus circulans GRS 313 [DOI]
- 20 Strategies for Improving Enzymes for Efficient Biocatalysis
- 21 Immobilization of α-amylase from mung beans (Vigna radiata) on Amberlite MB 150 and chitosan beads: A comparative study [DOI]
- 22 Immobilization of α-amylase on Cu2+ chelated poly(ethylene glycol dimethacrylate-n-vinyl imidazole) matrix via adsorption [DOI]
- 23 Sweet potato β-amylase immobilized on chitosan beads and its application in the semi-continuous production of maltose [DOI]
- 24 Activation of poly(dimer acid-co-alkyl polyamine) particles for covalent immobilization of α-amylase [DOI]
- 25 Immobilization of a thermostable α-amylase, Termamyl®, onto nitrocellulose membrane by Cibacron Blue F3GA dye binding [DOI]
- 26 Hydrolysis of soluble starch using Bacillus licheniformis α-amylase immobilized on superporous CELBEADS [DOI]
- 27 High Specific Activity of Raw‐Starch Digesting‐Glucoamylase Producing Rhizopus sp. A‐11 in Liquid Culture [DOI]
- 28 Purification and characterization of two raw-starch-digesting thermostable α-amylases from a thermophilic Bacillus [DOI]
- 29 Covalent immobilization of α-amylase onto poly(2-hydroxyethyl methacrylate) and poly(styrene -2-hydroxyethyl methacrylate) microspheres and the effect of Ca2+ ions on the enzyme activity [DOI]
- 30 Immobilization of α-Amylase from Bacillus circulans GRS 313 on Coconut Fiber [DOI]
- 31 Immobilization of glucoamylase via its carbohydrate moiety on macroporous poly(GMA-co-EGDMA) [DOI]
- 32 Reversible immobilization of glucoamylase by ionic adsorption on sepabeads coated with polyethyleneimine. [DOI]
- 33 Purification and some properties of a novel raw starch-digesting amylase fromAspergillus carbonarius [DOI]
- 34 Degradation of Raw Starch Granules by α-Amylase Purified from Culture of Aspergillus awamori KT-11 [DOI]
- 35 Evaluation of man-tailored cellulose-based carriers in glucoamylase immobilization [DOI]
- 36 Immobilization of glucoamylase and trypsin on crosslinked thermosensitive carriers [DOI]
- 37 Polyketone polymer: A new support for direct enzyme immobilization [DOI]
- 38 Immobilization of Geobacillus pallidus RAPc8 nitrile hydratase (NHase) reduces substrate inhibition and enhances thermostability [DOI]
- 39 Proteolysis of α-amylase from Saccharomycopsis fibuligera: characterization of digestion products [DOI]
- 40 Growth Profile and Amylolytic Activity of a Thermophilic Fungus Aspergillus fumigatus Isolated from Soil [DOI]
- 41 Influence of the immobilization chemistry on the properties of immobilized β-galactosidases [DOI]
- 42 Immobilization of α-amylase onto K-10 montmorillonite: characterization and comparison of activity in a batch and a fixed-bed reactor [DOI]
- 43 Raw Starch Degrading Amylase Production by Various Fungal Cultures Grown on Cassava Waste [DOI]
- 44 Stabilization of a raw starch digesting amylase from Aspergillus carbonarius via immobilization on activated and non-activated agarose gel [DOI]
- 45 Immobilization of Aspergillus niger Pectinase on Polyacrylonitrile Copolymer Membrane [DOI]
- 46 A newly isolated Rhizopus microsporus var. chinensis capable to secrete amyloltic enzymes with raw-starch-digesting activity [DOI]
- 47 Isolation of raw starch hydrolysing fungi and purification of α-amylase from Geotrichum candidum CMSS06 [DOI]
Cited by 10
Sandeep Mora, Sujit Banerjee · Industrial & Engineering Chemistry Research · 2013
Emmanuel Tobechukwu Ugwuoji, Tochukwu Nwamaka T. Nwagu, Lewis Iheanacho Ezeogu · Biotechnology Reports · 2023
Tochukwu Nwamaka Nwagu, Bartholomew Okolo, Hideki Aoyagi · Bioresource Technology Reports · 2021
Chika Jude Ugwuodo, Tochukwu Nwamaka Nwagu · International Journal of Biological Macromolecules · 2021
Guilong Yan, Yuzhen Zhou, Jianguo Wu · Applied Biochemistry and Biotechnology · 2023
Showing 5 of 10 known citations — external sources report more than can currently be individually listed.
Related research
- Effect of Carbohydrate Type on Brain Composition and Senescence in Aging, Hyperinsulinemia-prone OBESE LA/Ntul//-cp Rats — shares topic coverage
- Extracellular Amylases Produced by Bacillus thuringiensis — shares topic coverage
- Isolation, Identification and Optimization of Culture Conditions of Bacillus sp. Strain PM1 for Alkalo-thermostable Amylase Production — shares topic coverage
- Histological Study of the Polarity of Yam Tuber (Dioscorea spp.) at the Beginning of Tuberization — shares topic coverage
- Variance of Arrowroot (Maranta arundinacea) Starch Granule Morphology among Five Different Provinces in Sri Lanka — shares topic coverage
Article metrics
Real usage data collected on this platform.
77
Page views
0
PDF downloads
28
Outbound clicks
10
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.