Identification of Compounds Present in the Ethyl Acetate Fraction of Young Unopened Leaves of  Piliostigma thonningii by Liquid Chromatography–Mass Spectrometry

Thomas Konan Kouamé; Sorho Siaka; Amon Diane Marina N’Tamon; Yaya Soro

doi:10.9734/ijbcrr/2025/v34i2973

Research Article Open access CC BY 4.0

Identification of Compounds Present in the Ethyl Acetate Fraction of Young Unopened Leaves of Piliostigma thonningii by Liquid Chromatography–Mass Spectrometry

Thomas Konan Kouamé, Sorho Siaka, Amon Diane Marina N’Tamon, Yaya Soro

International Journal of Biochemistry Research & Review · pp. 154–167 · Published 18 Mar 2025

10.9734/ijbcrr/2025/v34i2973

Abstract

Piliostigma thonningii (Schumach Mile-Redh) is a species of the Fabaceae family. In Côte d'Ivoire, the young, unopened leaves of this species are used to treat certain pathologies. The aim of this study was to determine the compounds present in the ethyl acetate fraction of young, unopened leaves of Piliostigma thonningii. Successive fractionation of the hydroethanol extract resulted in the ethyl acetate fraction. LC-MS analysis identified seven (7) compounds in this fraction. These analyses revealed that young, unopened leaves of Piliostigma thonningii are rich in unidentified chemical compounds.

Piliostigma thonningii fabaceae LC-MS pathology

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Thomas Konan Kouamé, Sorho Siaka, Amon Diane Marina N’Tamon, Yaya Soro (2025). Identification of Compounds Present in the Ethyl Acetate Fraction of Young Unopened Leaves of Piliostigma thonningii by Liquid Chromatography–Mass Spectrometry. International Journal of Biochemistry Research & Review , 154–167. https://doi.org/10.9734/ijbcrr/2025/v34i2973

Affiliations

Thomas Konan Kouamé — Laboratoire des Procédés Industriels de Synthèse, de l’Environnement et des Energies Nouvelles (LAPISEN), Institut National Polytechnique Félix HOUPHOUËT-BOIGNY de Yamoussoukro, BP 1093 Yamoussoukro, Côte d’Ivoire.
Sorho Siaka — Laboratoire des Procédés Industriels de Synthèse, de l’Environnement et des Energies Nouvelles (LAPISEN), Institut National Polytechnique Félix HOUPHOUËT-BOIGNY de Yamoussoukro, BP 1093 Yamoussoukro, Côte d’Ivoire.
Amon Diane Marina N’Tamon — Département de Chimie Analytique, Minérale et Générale, Technologie Alimentaire, UFR Sciences Pharmaceutiques et Biologiques, Université Félix Houphouët-Boigny, 06 B. P. 2256 Abidjan 06, Côte d’Ivoire and Laboratoire de Pharmacognosie, BioCIS, Unix. Paris-Sud, CNRS, Université Paris-Saclay, Faculté de Pharmacie, 5 rue J.-B. Clément, 92290 Châtenay-Malabry, France.
Yaya Soro — Laboratoire des Procédés Industriels de Synthèse, de l’Environnement et des Energies Nouvelles (LAPISEN), Institut National Polytechnique Félix HOUPHOUËT-BOIGNY de Yamoussoukro, BP 1093 Yamoussoukro, Côte d’Ivoire.

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