Anti-proliferative Properties of New Variety Organic Rice MRQ74 Extracts against Colon Cancer Cells: In-vitro Study

Abstract

Aims: To examine the anti-proliferative properties of different extracts of new variety an organic rice MRQ74 towards colon cancer cells: in-vitro study. Study Design: Experimental study. Place and Duration of Study: Central Laboratory, Tissue Culture Laboratory, University of Sultan Zainal Abidin, Terengganu from November 2019 until February 2020. Methodology: The organic rice MRQ74 extracts had been led to tetrazolium salt reduction (MTT) assay and an inhibition concentration of 50 (IC50) value for their cytotoxic potential against colon cancer cells. Meanwhile, cells morphology observation and fluorescence double staining of treatment cells were determined using a light inverted microscope and acridine orange/propidium iodide staining. Results: Results showed that 50% aqueous ethanol of rice grains gave the lowest IC50 values towards HCT-116 and CT-26 cell lines, while an aqueous solution of rice grains gave the lowest IC50 values towards HT-29 cells (p<0.05). Thoroughly, the treated colon cancer cells had shown morphological alterations after treated with different solvent extracts of an organic rice MRQ74. Conclusion: The outcomes had observed preliminary results on cancer study for better health by inspiring the consumption of an organic rice MRQ74 and future product developments.