Genomic Investigation of bla-PAO and bla-OXA50 in Multidrug-Resistant Pseudomonas aeruginosa from Clinical Samples in Abeokuta, Ogun State, Nigeria

Abstract

Pseudomonas aeruginosa is one of the pathogens of human concern with high intrinsic multi-drug resistance capabilities. The genomic investigation of blaPAO and blaOXA-50 was done on multi drug-resistant Pseudomonas aeruginosa that were also resistant to carbapenem among the isolates collected from a total of 128 clinical samples in Abeokuta, Nigeria. Pseudomonas aeruginosa isolates were obtained from pure culture, and profiled for antibiogram by disc diffusion method. Genomic DNA from isolates were typed for blaPAO and blaOXA-50 with PCR. A total of 75 samples (58.6%) yielded the growth of bacterial isolates. Bacteria isolated were Escherichia coli (18.8%), Klebsiella pneumoniae (16.4%), Staphylococcus aureus (8.6%), Pseudomonas aeruginosa (7.8%), Streptococcus pnuemoniae (3.1%), Proteus mirabilis (2.3%), and Enterobacter aerogenes (1.6%). Only ten (30%) isolates were confirmed to be Pseudomonas aeruginosa. All the P. aeruginosa isolates were resistant to ampicillin, cloxacillin, erythromycin, and tetracycline. Out of these ten multidrug Pseudomonas aeruginosa isolates, only three (30%) were resistant to carbapenems. Only two of these isolates expressed blaOXA-50 and blaPAO, while one possessed only blaPAO. Close Continuous monitoring of these antibiotic-resistant pathogens and hospital surveillance needs to be adopted to reduce their spread to other healthcare facilities.