Aggregative Activity of Basic Regular Blood Elements and Vascular Disaggregating Control over It in Calves of Milk-vegetable Nutrition

Abstract

Estimation of ontogenetic peculiarities of aggregation of regular blood elements and vascular control over it, including calves in the middle of early ontogenesis - in the phase of milk-vegetable nutrition, is of great scientific and practical interest. Clarification of their physiological balance is really significant for receiving the possibility of regulation of hemostasis activity and metabolism intensity in tissues. To estimate aggregative activity of regular blood elements and disaggregating vessels' control over them in calves during the phase of milk vegetable nutrition. We formed a group of calves of black-many- colored breed which were examined 5 times at the age between the 31st and the 90th days of life with the application of biochemical, hematological and statistical methods of investigation. Farm "Kolos" of Fatezh district, Kursk region, Russia, spring 2014. The work was conducted with the help of 41 calves of black many-colored breed taken into investigation on the 31st day of life. Examination was conducted on the 31st, 45th, 60th, 75th and 90th days of calves' lives. We estimated the intensity of lipids' peroxidation in plasma, aggregation of erythrocytes, platelets and neutrophils in plasma received without application of a cuff on a vessel and with its usage. The calves of 45 days of life were noted to have short-period increase of summary erythrocytes' quantity in aggregate (on 15.9%) and aggregates' quantity (on 13.4%) at decrease of free erythrocytes (on 9.7%). Given indices returned in calves to the initial level by the 90th day of life. In plasma after temporary venous occlusion in calves by the 45th day of life summary erythrocytes' quantity in aggregates (on 21.7%) and aggregates' number (on 16.3%) had a short-term increase at decrease of free erythrocytes on 13.9%. By the 45th day of life their time of platelets' aggregation development with collagen shortened to 22.5±0.12s returning by the end of investigation to the initial level. In plasma after temporary venous occlusion we found peak acceleration of platelets' aggregation by the 45th day of life. The calves of 45days of life were noted to have strengthening of neutrophils' aggregation with lectin on 10.1%, concanavalin A - on 9,4%, phytohemagglutinin – on 19.1% eliminated by the 90th day of life. Found in calves of 45 days of life short-period strengthening of formular blood elements' aggregation was combined with brief weakening of their deaggregating vascular activity.