Isolation, Characterisation and Antimicrobial Activity of Chemical Constituents from the Root of Mezoneuron angolense (Caesalpiniaceae)

Abstract

Aims: In Ivory Coast, Mezoneuron angolense roots are well-known in traditional medicine for their efficiency in the treatment of diarrhoea. The goal of this study was to determine the antibacterial constituents of these roots. Methodology: The chemical investigations of the roots of the plant have been undertaken and the structure of isolated compounds was elucidated through spectral studies including IR, UV, MS, 1D-NMR (1H and 13C NMR) and 2D-NMR experiments (HSQC, HMBC, COSY, and NOESY). The isolated compounds were screened against three enteropathogenic bacteria, Vibrio cholerae, Salmonella typhi and Shiguella fleneii, using microbroth dilution method. Results: These investigations conducted to the isolation of two sterols, two stilbenes, one phthalate, and one carbohydrate. All tested compounds except Bis (2-methylheptyl) phtalate (2) were active against all pathogens with MICs and MBCs ranging from 312.50 to 625 mg/ml. Piceatannol (5) and Trans-resveratrol (3) were the most active compounds. Conclusion: The results from the current study confirm and justify the popular use of the roots of this plant in the treatment of infectious diarrhoea. All obtained compounds were isolated from this species for the first time.