Physiological Peculiarities of Vessels’ Disaggregating Control over New-Born Calves’ Erythrocytes

Abstract

Introduction: The process of microcirculation determines significantly the common physiological status of calves including the new-born phase. The success of microcirculation process in tissues mostly depends on aggregation evidence of the most numerous population of regular blood elements – erythrocytes and the level of vascular wall’s disaggregating control over them. The aim is to determine peculiarities of vascular control over erythrocytes’ aggregation in new-born calves. Materials and Methods: The study was conducted on 32 calves of black-multicolored breed. All the calves were healthy and were got from healthy cows after normally progressing pregnancy. All the animals were kept on the territory of Central Russia. They were examined on the 1st-2nd, 3rd-4th, 5th-6th, 7th-8th and 9th-10th days of ontogenesis. Lipid peroxidation in plasma was estimated by standard methods according to the amount of thiobarbituric acid-active products and the level of acylhydrazines with the account of plasma antioxidant activity. Spontaneous aggregation of erythrocytes and its response to temporary venous occlusion were also estimated. A single-factor analysis of variance was used with application of Fisher’s F-reliability criterion. Differences in data were considered to be statistically significant at р<0.05. Results: In the course of the new-born phase the calves were noted to have a weak trend to strengthening of initially low erythrocytes’ aggregative activity (the number of erythrocytes’ aggregates on the 1st-2nd day was equal to 8.0±0.14, on the 9th-10th day – 8.2±0.19). The presence of inactive erythrocytes’ aggregation in new-born calves was mostly provided by strong disaggregating vessels’ impact on them (the index of vessels’ control over the quantity of erythrocyte aggregates on the 1st-2nd day was equal to 1.11±0.004, on the 9th-10th day – 1.17±0.003). Found level of accountable indices is mostly connected with low activity of examined animals’ plasma peroxidation (acylhydroperoxides on the 1st-2nd day were equal to 1.53±0.26 D233/1 ml, on the 9th-10th day – 1.42±0.31 D233/1 ml), what damaged weakly the membranes of erythrocytes and endotheliocytes. Conclusion: New-born calves are characterized by physiologically favorable for microcirculation balance of erythrocytes’ aggregation and vascular wall’s disaggregating control over it. Received values of hematological indices can be useful for further researches of new-born calves’ physiology.