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Research Article Open access CC BY 4.0

Fluorescent Detection of Specific DNA Sequences Related to Toxoplasma gondii Based on Magnetic Fluorescent Nanoparticles Fe3O4/CdTe Biosensor

Lei He, Liusong Ni, Xueping Zhang, Chen Zhang, Shichao Xu, Runlan Li

International Journal of Biochemistry Research & Review · pp. 130–139 · Published 2 Feb 2015

10.9734/IJBCRR/2015/15254

Abstract

Fluorescent Detection of Specific DNA Sequences Related to Toxoplasma gondii Based on Magnetic Fluorescent Nanoparticles Fe3O4/CdTe Biosensor Magnetic fluorescent nanoparticles (Fe3O4/CdTe) were prepared in this work and applied for Toxoplasma gondii DNA detection. First, CdTe quantum dots were synthesized with 3-mercaptopropionic (MPA) capped. Fe3O4 magnetic particles were prepared by hydrothermal method with NaOH as precipitator, and they were surfacely modified with silane coupling agent (KH550). After then, the MPA-capped CdTe QDs were immobilized on the Fe3O4 particles surface via electrostatic interaction, and the Fe3O4/CdTe particles were prepared with the average size of 10 nm. The DNA sensing probe was fabricated through labeling a stem-loop Toxoplasma gondii DNA oligonucleotides with Fe3O4/CdTe (donor) at the 5′ end and BHQ2 (acceptor) at 3′ end, respectively. The assembly prosess was verified by UV-Vis, TEM, IR, XRD etc. The sensitivity characterization of the molecular beacon probe was performed by fluorescence spectrum (FS) with a detection limit of 8.339x10-9M. This chemical strategy can be further applied to prepare the magnetic nanoparticles for DNA detection.

Fe3O4/CdTe nanoparticles molecular beacon probe fluorescence resonance energy transfer Toxoplasma gondii DNA detection

References (8)

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Cited by 27

Development of direct assays for Toxoplasma gondii and its use in genomic DNA sample

Lívia M. Alves, Vinícius R. Rodovalho, Ana C.H. Castro · Journal of Pharmaceutical and Biomedical Analysis · 2017

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