Geno-Protective, Free Radical Scavenging and Antimicrobial Potential of Hyptis suaveolens Methanolic Fraction: An In-Vitro Study

Abstract

Aims: Hyptis suaveolens (L.) Poit, is one of the natural herbs with several medicinal properties. However, many medicinal aspects of this herb still need to be explored. Therefore, our aim was to examine the antioxidant, antimicrobial properties and genoprotective effect of H. suaveolens methanolic extracts (HSME) of seed, stem, and root. Study design: extraction and therapeutic aspects of H. suaveolens. Place and Duration of Study: 1) Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al-Majmaah and 2) Clinical Biochemistry & Natural Product research laboratory, Department of Biosciences, Integral University, lucknow between 2018-2020. Methodology: HSME were extracted through soxhlet extractor and further analyzed for TPC, antioxidant activity through DPPH and FRAP assay followed by antimicrobial potential through zone of inhibition and MIC/MBC assay. We also examined the genoprotective properties of HSME on oxidative DNA damage. Results: Our results showed that TPC (180±5 mg GAE/g dw), DPPH scavenging activity (IC50 value = 72±0.45 µg/ml) and FRAP value (1.443±0.02 µM ferrous ion/mg extract) was highest in HSME seeds followed by root and stem. The results also illustrated that the antimicrobial activity of HSME (seed and stem) against five bacterial strain were found very effective than root part. Moreover, genoprotective effect of HSME seeds (80±3 % retention) was better than stem (41±2 %) and root (32±2 %) extract. Conclusion: The study revealed that HSME seed extract showed potential bioactivities might be due to presence of high TPC and can be used to treat diseases related with oxidative stress or microbial infections.