Effects of Lead Acetate Toxicity in Respect to Histological, Cytogenetical, Haematological Parameters and Subsequent Amelioration by Green Tea on Experimental Albino Rats

Abstract

Lead acetate is arguably one of the environmental pollutants that threats the lives of living beings in multiple ways. Lead acetate occurs both naturally & due to human activities. Lead Acetate, a potential carcinogen, can enter the body through inhalation, ingestion, or dermal contact. Green tea and polyphenol have recently drawn attention of cell biologists. Multiple studies have confirmed the anti-inflammatory, anti-oxidative & anti-diabetic activities of polyphenols. In the Green tea extracts, Catechins including EGC (Epigallocatechin), ECG (Epicatechin-3-gallate), EGCG (Epigallocatechin-3-gallate) and EC (Epicatechin) are the major polyphenols which show a vast range of ameliorating effects on deleterious effects of different organs. Proper supplementation or dosage of green tea extracts may improve TAC (Total antioxidant capacity) and reduce the concentration of MDC (Malondialdehyde) in liver, brain and blood of vertebrates. Epicatechin supplementation could significantly lower the MDA levels and increase the glutathione concentration & catalase. It also increases superoxide dismutase (SODs, helpful antioxidant to defense against oxidative stress) & glutathione peroxidase activities in major organs. Though, high doses might have adverse effects, it has the capability to protect against oxidative damage & promote overall health.  Twenty mature albino rats were used, divided into four groups of five rats per group. Group 1 (control male) were not administered with lead acetate or green tea extract; it received water only. Group 2 (male) was given lead acetate solution 0.003 ml/g; Group 3 (female) was administered with lead acetate 0.003 ml/g and Group 4 (male) was treated with lead acetate (0.003 ml/g) followed by green tea extract (0.0002 ml/g) daily. This study investigated the effect of lead acetate intoxication and ameliorating upshot of green tea extract on some haematological, histological, cytogenetical factors of Albino Rats.