A Novel Test System for Genotyping rs43703016 Single-nucleotide Substitutions in the Bovine CSN3 Gene
Svetlana Kovalchuk, Arina Tagmazyan, Eugene Klimov
Annual Research & Review in Biology · pp. 1–5 · Published 30 Jul 2019
10.9734/arrb/2019/v32i430090Abstract
Aims: Caseins are among the main milk proteins that determine many of its properties. Bovine kappa-casein (CSN3) is associated with the qualitative composition of milk, as well as with the quality of cheese obtained from this milk. The rs43703016 single-nucleotide substitution (g.88532332A>C; Asp148Ala) in exon 4 of the bovine CSN3 gene plays an important role in the production of quality hard cheeses. Various methods for the DNA testing of this substitution have been developed in the last three decades. Emergent DNA technologies provide an opportunity to modernize methods of genotyping single-nucleotide polymorphisms. Results: We have developed and verified a method to differentiate A/C alleles of the rs43703016 substitution in the bovine CSN3 gene by real-time PCR using allele-specific fluorescent probes. Conclusion: Our new method allows fast genotyping of animals, and may be used for selection of cows carrying the CC genotype, which determines good cheese-making properties of milk.
Cited by 2
R. R. Vafin, Kh. Kh. Gilmanov · SERIES CHEMISTRY AND TECHNOLOGY · 2021
L. Jiménez-Montenegro, J. A. Mendizabal, L. Alfonso · Scientific Reports · 2022
Related research
- Prevalence of Enteropathogenic Escherichia coli in Children under 5 Years with Diarrhoea in Yola — shares topic coverage
- Recent Advances in Human Papillomavirus Detection and Genotyping — shares topic coverage
- Prevalence and Molecular Diversity of Legionella pneumophila in Domestic Hot Water Systems of Private Apartments — shares topic coverage
- Multi-locus Genotyping Reveals High Occurrence of Mixed Assemblages in Giardia duodenalis within a Limited Geographical Boundary — shares topic coverage
- First Molecular Characterisation of Clinical Strains of Mycobacterium ulcerans in Togo (West Africa) — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
2
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.