Optimization of Water Based-extraction Methods for the Preparation of Bioactive-rich Ginger Extract Using Response Surface Methodology
K. D. P. P. Gunathilake, H. P. Vasantha Rupasinghe
European Journal of Medicinal Plants · pp. 893–906 · Published 12 May 2014
10.9734/EJMP/2014/10322Abstract
Aims: Evaluation of three extraction methods to prepare bioactive-rich ginger extract for incorporation into a functional beverage. Study Design: Response surface methodology. Methodology: For the preparation of bioactive-rich ginger extract with water, conventional hot water extraction, ultrasonic-assisted extraction and high pressure homogenization-assisted extraction were evaluated. Response surface methodology was employed to optimize the extraction conditions of each method with respect to the highest polyphenols, antioxidant capacity (ferric reducing antioxidant power; FRAP) and percent inhibition of low density lipoprotein (LDL) cholesterol oxidation. Results: Multiple response optimizations revealed that the optimum extraction conditions for each extraction method were 60min extraction time under 55°C for hot water extraction, 15min ultrasonication under 52°C for ultrasonic-assisted extraction and 62°C under 140MPa homogenization pressure for high pressure homogenization-assisted extraction. Conclusion: The extract prepared from the ultrasonic-assisted extraction method exhibited the highest polyphenol recovery and antioxidant activity, compared to the extracts prepared from other two methods.
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