Isolation and Characterization of Plant Growth Promoting Rhizobacteria Enterobacter hormaechei and their Suppression Efficacy against Colletotrichum falcatum in Combination with Chitosan
Deepmala Katiyar, A. Hemantaranjan, Bharti Singh, Abhishek Kumar Malakar
International Journal of Plant & Soil Science · pp. 1–12 · Published 7 Feb 2017
10.9734/IJPSS/2017/31549Abstract
Aims: This study aimed to explore the suppression efficacy of plant growth promoting rhizobacteria (PGPR) Enterobacter hormaechei, chitosan and its oligomers either singly or in combination on red rot disease causing pathogen Colletotrichum falcatum in sugarcane. Methodology: The study was conducted to isolate twenty nine bacteria from sugarcane rhizosphere and investigate their potential for plant growth activities. Selected isolate PSC3 was characterized by biochemical and molecular identification by 16S rRNA sequencing. The study was further preceded for in vitro screening of plant growth promoting traits viz., production of Indole-3-acetic acid (IAA), hydrogen cyanide (HCN) ammonia (NH3) production and antifungal activity against C. falcatum. Results: Among twenty nine isolates strain PSC3 showed highest plant growth promoting traits viz., indole-3-acetic acid, hydrogen cyanide, ammonia production and antifungal activity against C. falcatum among other isolated strains. Nucleotide 16S rRNA sequence analysis using clustalW program revealed that isolate PSC3 showed phylogenetic affiliation and maximum homology (99%) with E. hormaechei. Antifungal activity of chitosan, chitooligosaccharides (COS) and E. hormaechei were checked by inhibition of C. falcatum mycelial radial growth. Among three treatments of chitosan, COS and chitosan + E. hormaechei, two treatments showed significant antifungal activity (P<0.05). Chitosan treatment showed radial growth range from 2.5±0.07 to 1.9±0.03 cm against C. falcatum in comparison with control (9.1±0.09 cm). The significant growth inhibition 79.0% was observed in chitosan at concentration 0.6% but the combination of chitosan with PGPR E. hormaechei PSC3 showed highest growth suppression of C. falcatum (86.8%) whereas fungal treated with only E. hormaechei showed growth radial inhibition 41.3%. Conclusion: The findings reveal that chitosan and E. hormaechei have significant effect on C. falcatum. This new antifungal combination may be help to prevent red rot disease in sugarcane.
Cited by 0
No indexed citations yet.
Related research
- Susceptibility of Some Clinically Resistant Bacterial Isolates to Thyme Essential Oil, Chitosan and Lactobacillus reuteri — shares topic coverage
- Effects of Addition of TiO2 Edible Coatings and Storage Periods on the Chicken Eggs Quality — shares topic coverage
- Gelatin, Chitosan, and Beeswax Based Edible Packaging for Food Product: A Review — shares topic coverage
- Functional Edible Coating from Banana Peel Starch (Musa sapientum L.) and Vannamei Shrimp Shell Chitosan for Quality Maintenance of Tomato — shares topic coverage
- Effect of the Different Surface Coatings and Packaging Materials for Shelf Life and Quality of Guava (Psidium guajava L.) CV. Allahabad Safeda — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
0
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.