Anti-inflammatory Activity, GC-MS Profiling and in silico ADMET Analysis of Piper hymenophyllumMiq. Extracts as a Candidate for Gastrointestinal Disease Management

Abstract

The Piper genus, within the Piperaceae family, comprises over a thousand species recognized for their significant medicinal properties, especially in gastrointestinal health. This study investigates Piper hymenophyllum, a newly discovered wild species, focusing on its phytochemical profile, bioactivity and therapeutic value.GC-MS analysis identified major bioactive compounds such as Phytol, Cyclobutane,1,1,2,3,3-pentamethyl-, n-Hexadecanoicacid, gamma. Sitosterol, n-Tetracosanol-1, Stigmasterol, l-Valine, n-pentafluoropropionyl-, isobutyl ester, Octadecanoic acid, 2,3-dihydroxypropyl ester, Octahydroxanthen-1,9-dione, 3,3,7,7, -tetramethyl-10-hexyl, 1- Pentanone, (4- methoxyphenyl) - oxime which exhibit potent antioxidant and antimicrobial effects relevant to gastroprotection. The ADMET analysis confirmed favorable pharmacokinetic and safety profiles for these key compounds. Experimental findings demonstrated that extracts of P. hymenophyllum effectively alleviate symptoms of gastrointestinal disorders and display promising anti-Inflammatory Bowel Disease (IBD) activity, indicating its potential as a multi-target natural therapeutic for IBD management.