Evaluation of the Growth of G6 Transgenic Mutiara Catfish (Clarias gariepinus) after Mixed Feeding of Snail Flour (Achatina fulica) and Commercial Feed
Ibnu D. Buwono, Roffi Grandiosa. H, Iskandar, Putri Budi. S
Asian Journal of Biotechnology and Genetic Engineering · pp. 173–184 · Published 13 Jul 2024
Abstract
This research was conducted in Hatchery Building 4, Faculty of Fisheries and Marine Sciences, Padjadjaran University. The purpose of this experiment was to obtain the optimal ratio of snail flour mixture and commercial feed (Hi Pro Vite 781) to support growth, feed efficiency ratio, protein retention value, and carbohydrate retention in G6 transgenic mutiara catfish. The study was carried out experimentally with four treatments and three replicates (CRD), the treatment given was the ratio of commercial feed mixture: snail flour, namely A (60%: 40%), B (40%: 60%), C (20%: 80%) for G6 transgenic mutiara catfish and A* treatment (60%: 40%) for non-transgenic catfish (sangkuriang catfish as a control). Significant differences between treatments were tested using the Duncan Multiple Range Test (DMRT) with a confidence level of 95%. Data on absolute weight growth (Wg), feed conversion ratio (FCR), protein efficiency ratio (PER), protein retention, and carbohydrate retention were analyzed using Sigmaplot 15.0. The results showed that treatment B using 40% snail flour showed the highest body weight gain (402.357 g), lowest FCR (0.69), protein efficiency ratio (16.91%), best protein retention (53.01%) and lowest carbohydrate retention (11.32%) compared to other treatments. The best results in treatment B were due to Transgenesis-GH inducing a protein sparing effect, showing that the efficiency of feed use and protein use in transgenic G6 mutiara catfish increased the conversion of mixed feed carbohydrates of treatment B into protein. As a consequence, the growth of transgenic catfish is increased higher than that of non-transgenic fish, which is indicated by increased retention value and protein efficiency.
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