Skip to content
Research Article Open access CC BY 3.0

Optimization of Sweet Potato Starch Hydrolyzate Production and Its Potential Utilization as Substrate for Citric Acid Production

E. Betiku, O. A. Adesina

Biotechnology Journal International · pp. 169–182 · Published 23 Feb 2013

10.9734/BBJ/2013/2768

Abstract

Aims: The aims of this work was optimization of two-step enzymatic hydrolysis of sweet potato starch using statistical approach and subsequent utilization of the hydrolyzate obtained for citric acid production. Methodology: Box Behnken design was used in this study to generate a total of 17 individual experiments for each step of the hydrolysis (liquefaction and saccharification steps). These were designed to study the effect of temperature, time and pH on the sweet potato starch hydrolyzate (SPSH) concentration. The optimization was carried out using response surface methodology (RSM). The SPSH obtained was used to culture Aspergillus niger for citric acid production. Results: A statistically significant quadratic regression model (P<0.05) was obtained for the liquefaction step. Statistical model predicted the highest sweet potato starch hydrolyzate (SPSH) concentration to be 172.23 g/L at optimal condition of temperature 61.05ºC, time 55.02 min and pH 6.5. A statistically significant quadratic regression model was also obtained for the saccharification step. Statistical model predicted the highest SPSH concentration to be 241.92 g/L, established at the optimal condition of temperature 52ºC, time 44 min and pH 4.5. The optimal liquefaction and saccharification conditions were validated with the actual SPSH concentration of 172.00 and 241.01g/L, respectively. The maximum citric acid production of 86g/L was achieved on the 8th day of cultivation when the SPSH was used for the cultivation of A. niger. Conclusion: RSM was successfully applied the two-step enzymatic hydrolysis of sweet potato starch. This work showed that the sweet potato starch hydrolyzate could serve as sole carbon source for citric acid production.

Enzymes optimization response surface methodology sweet potato Aspergillus niger cultivation citric acid

References (18)

  1. 1 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar [DOI]
  2. 2 Advances in microbial amylases [DOI]
  3. 3 Direct Determination of Citric Acid in Milk with an Improved Pyridine-Acetic Anhydride Method [DOI]
  4. 4 New Perspectives for Citric Acid Production and Application
  5. 5 Optimization of Viscozyme L-assisted extraction of oat bran protein using response surface methodology [DOI]
  6. 6 Enzymes involved in the processing of starch to sugars [DOI]
  7. 7 Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature [DOI]
  8. 8 Acid hydrolysis of sweet potato for ethanol production [DOI]
  9. 9 Optimization of Ethanol Fermentation from Sweet Sorghum Juice Using Response Surface Methodology [DOI]
  10. 10 Enzymatic hydrolysis optimization of sweet potato (Ipomoea batatas) peel using a statistical approach [DOI]
  11. 11 Alcohol fermentation of sweet potato. I. Acid hydrolysis and factors involved [DOI]
  12. 12 Citric acid production from the mash of dried sweet potato with its dregs by Aspergillus niger in an external-loop airlift bioreactor [DOI]
  13. 13 Biotechnological potential of industrial wastes for economical citric acid bioproduction by Aspergillus niger through submerged fermentation [DOI]
  14. 14 Alcohol fermentation of sweet potato. Membrane reactor in enzymatic hydrolysis [DOI]
  15. 15 A comparison of the hydrolysis of sweet potato starch with β-amylase and infrared radiation allows prediction of reducing sugar production [DOI]
  16. 16 Citric acid fermentation of hydrolysed raw starch by Aspergillus niger IIB-A6 in stationary culture.
  17. 17 Nitrogen-dependent regulation of gluconic and/or citric acid production by Aspergillus niger
  18. 18 Appendix 2: Selected Excerpts from the Food and Agriculture Organization (FAO) of the United Nations Code of Conduct for Responsible Fisheries [DOI]

Cited by 12

Applying the Response Surface Methodology (RSM) Approach to Predict the Tractive Performance of an Agricultural Tractor during Semi-Deep Tillage

Mohammad Askari, Yousef Abbaspour-Gilandeh, Ebrahim Taghinezhad · Agriculture · 2021

Assessment of adaptive neuro-fuzzy inference system and response surface methodology approaches in draft force prediction of subsoiling tines

Mohammad Askari, Yousef Abbaspour-Gilandeh · Soil and Tillage Research · 2019

Mathematical modeling of starch gelatinization and some quality properties of parboiled rice based on parboiling indicators using RSM

Ebrahim Taghinezhad, Tom Brenner · Journal of Food Process Engineering · 2016

History, Evolution and Future of Starch Industry in Nigeria

Obi Peter Adigwe, Judith Eloyi John, Martins Ochubiojo Emeje · Biochemistry · 2022

Showing 4 of 12 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

48

Page views

0

PDF downloads

23

Outbound clicks

12

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.