Multidrug Resistance Pattern of Salmonella Typhimurium Isolated from Rectal Swabs of Stray Dogs at Chittagong Metropolitan Area (CMA), Bangladesh

Abstract

A cross-sectional study was conducted to investigate the prevalence and antimicrobial resistance pattern in Salmonella from 108 rectal swab of stray dogs of 9 randomly selected areas of Chittagong Metropolitan Area. Rectal swabs were collected for isolating Salmonella enterica serovar Typhimurium through bacteriological culture and InvA specific PCR assay followed by antimicrobial susceptibility testing. Out of the 108 samples, females showed higher prevalence (66.67%) than males (58.93%). Among the 67 bacterial culture positive isolates, 10.45% was Salmonella Typhimurium in InvA gene specific PCR. Isolated Salmonella was tested for resistance to twelve different antimicrobial agents, using disc diffusion method. In cultural sensitivity test, 100% resistance was found against Amoxicillin where higher resistance was found against Azithromycin, Cefixime, Ampicilin and Pefloxacin. Gentamycin and Colistin appeared to be sensitive. Multidrug resistance of Salmonella spp. has increased with a great deal in developing countries in the last decades. In this study, most of the Salmonella isolates were multidrug resistant. Rational use of antibiotics needs to be adopted in clinical practice to prevent the emergence of multi-drug resistance Salmonella and their zoonotic transmission.