In-vitro Antioxidant and Radical Scavenging Potentials of Artocarpus heterophyllus Seed Extract

Abstract

The study investigated the scavenging potentials of Artocarpus heterophyllus seed extract on DPPH, hydroxyl radical, superoxide anion radical, inhibition of nitric oxide formation, inhibition of non-enzymatic lipid peroxidation and reduction of ferric ion compared to various standards following standard procedures. The extracts and ascorbic acid showed potent antioxidant activity in DPPH radical scavenging assay (EC50= 1.53µg/ml) and (EC50 = 1.67µg/ml) and inhibited hydroxyl radical (EC50 = 1.20µg/ml) and (EC50 = 1.16µg/ml) respectively. Similarly, inhibited superoxide anion radical (EC50 =1.52µg/ml) and (EC50 =1.16µg/ml), inhibited formation of nitric oxide (EC50 = 1.44µg/ml) and (EC50= 1.71µg/ml), also inhibited non-enzymatic lipid peroxidation (EC50 = 1.81µg/ml) and (EC50 = 2.44 µg/ml) respectively. The extracts also reduced ferric ion Fe3+ to ferrous ion Fe2+. These results shows that ascorbic acid and butylated hydroxyl toluene (BHT) scavenged these radicals more than the extract. The effective radical scavenging properties of A. heterophyllus seed ehanol extract compared to the standards could be linked to the various phytochemical it contains and could also justify its use in the treatment of oxidative stress related diseases.