Control of the MAOA-L Allele Expression through Genetic Manipulation Techniques Using CRISPR/Cas9 System

Abstract

Antisocial behaviour is antagonism behaviour. It is a behavioural disorder inherited according to X-linked chromosome inheritance pattern. This disorder comes from mutations in the MAOA gene. One of the mutations in this gene triggers the MAOA-L allele activity. The MAOA-L allele activity can trigger the expression of antagonistic actions in both healthy and unhealthy people. Maltreatment of healthy boys during childhood can lead to antagonistic actions in these children. In animal models, MAOA inhibitors can reverse the expression of antagonistic actions to healthy behaviour. Currently, this disorder in humans cannot be suppressed permanently. In the future, to suppress behavioural disorder, technologies such as end-joining homology techniques, ssODN, iPSCs and CRISPR/Cas9 system might be performed. End-joining homology, ssODN or iPSCs in combination with CRISPR/Cas9 system has succeeded to edit mutant segments in the F8 gene or F9 gene. Both genes, as well as the MAOA gene, are located in the X-chromosome. It shows that the iPSCs, ssODN or HMEJ method in combination with CRISPR/Cas9 system can significantly help the suppression of MAOA-L allele expression which triggers antagonistic actions in humans.