Relation of Sirtuin 1 Gene Polymorphisms with Lipid Profile in Hemodialysis Patients

Abstract

Relation of Sirtuin 1 Gene Polymorphisms with Lipid Profile in Hemodialysis Patients Very little is known about the genetic variation of SIRT 1 and its effects on energy homeostasis in humans. Mammalian SIRT1deacetylates a host of target proteins that are important for apoptosis, the cell cycle, circadian rhythms, mitochondrial function and metabolism. In particular, much current research focuses on the impact of SIRT1 in glucose homeostasis, lipid metabolism and energy balance. Objective: To study the relationship of sirtuin 1 gene polymorphisms with lipids profile in hemodialysis patients. Subjects and Methods: This study included 70 Egyptian subjects (45 patients on hemodialysis and 25 age and gender matched healthy control group). The genotyping of SIRT1 rs7895833 in the promoter region, rs7069102 in intron 4, and rs2273773 in exon 5 was performed using polymerase chain reaction with confronting two-pair primers assay (CTPP). Serum TC, TG, HDLc, LDLc, fasting glucose, urea and creatinine were measured by standard colorimetric methods. Results: The patients had higher diastolic and systolic BP (P<0.001), fasting blood glucose (P<0.001), TC (P<0.001), TG (P=0.006), LDLc (P=0.004), urea (P<0.001) and creatinine (P<0.001). Males and female patients differ according to cause of hemodialysis (P= 0.02) and serum creatinine (P=0.007). Control subjects of sirtuin1 rs7895833 showed significant A allele compared with patients (46% vs. 27.78% P= 0.04) while C allele of sirtuin1 rs7069102 not differ between groups (P>0.05). Sirtuin 1 rs2273773, patients showed significant lower frequency of C allele compared with control (26.67% vs.44% P= 0.04,). Conclusion: Significant association of SIRT1 rs7895833 and rs2273773 polymorphisms with dyslipidemia and blood pressure may modulate disease course in hemodialysis.