Effect of Mutation on Seed Yield per Plant and Chlorophyll Content in M3 Generation of Green Gram [Vigna radiata (L.) R. Wilczek]

Abstract

Aims: Mutation breeding used to induce quantitative variability in M3 generation of green gram of two excellent variety GM-4 and Meha with a view to study effect various mutagen and for the estimation of genetic variability parameters like GCV, PCV, Heritability and Genetic advance percent of mean for characters like as seed yield per plant and chlorophyll content. Place and Duration of Study: The Field experiment was conducted at Instructional Farm, COA, Junagadh Agricultural University, Junagadh during the Summer-2015. Methodology: The experimental material consisted of M2 derived M3 seeds which consists of sixty-six M3 progeny lines (64 mutant + 2 controls). The progeny lines were selected on the basis of yield and its associated characters from M2 generation. Results: The analysis of variance between family revealed that families differed significantly for chlorophyll content while non-significant for seed yield per plant. GCV and PCV were higher in all the mutagenic family with respect to control for both the characters as well as for both the variety. For both characters, phenotypic coefficient of variation was higher than genotypic coefficient of variation indicating that there was environmental influence on these traits. Heritability exhibited higher in all the mutagenic families and also higher genetic advance was observed. The combined results for heritability showed that the high estimates of heritability and genetic advance were scored for seeds per plant indicating that these characters were under the control of additive genetic effects. Conclusion: Mutagenic progenies with additive gene effect can be selected for improvement of desirable traits in mungbean.