Effects of Cytokinins on in vitro Culture of Bauhinia purpurea L.

Abstract

Bauhinia purpurea L. is a moderate-sized tree with multipurpose value tree yields gum, its bark contains tannin and leaves are used as fodder. It is distributed in sub-Himalayan tracts. The main objective of this study was to evaluate the effects of different cytokinins on growth parameters of B. purpurea and develop a standard micropropagation protocol for nodes and shoot proliferations. The cytokinins used in this study were N-Benzyl-9(2-tetrahydropyranyl) (BPA), 6-furfurylaminopurine, Kinetin (Kn), 6-(4-Hydroxy-3-methyl-trans-2-butenylaminopurine) (Zeatin) (Zin), 2-isopentenyl aminopurine, (2-iP) and 6-benzylaminopurine (BAP) at four different concentrations (0.5, 1.0, 2.0 and 5.0 µM). Murashige and Skoog (1962) (MS) medium was used for the experimental purpose. Multiplication rate of plants was recorded after 8 weeks of culture. Such propagated best grown plants were acclimatized and transferred to the field. All the collected data were worked out statistically with SPSS, a system of analytical procedure