Comparative Analysis of Wet Mount and Concentration Methods for Detecting Intestinal Parasites in Fecal Specimens

Abstract

Background: Intestinal parasitic infections continue to pose a significant public health challenge, particularly in low-resource countries. Accurate laboratory diagnosis plays a vital role in initiating timely treatment and preventing transmission. Objective: This study aimed to assess and compare the diagnostic efficiency of direct saline/iodine wet mount and the Formol–Ether Concentration (FEC) method in stool examination. Methods: This 12-months 2022-2023 observational study analyzed 350 stool samples using both saline/iodine wet mount and FEC methods at the Department of Microbiology, School of Medical Science $ Research, Sharda University, Greater Noida -201306, Uttar Pardesh, India. Results: Of the 350 samples, 41 (12%) tested positive. The FEC method detected all 41 cases, while the wet mount identified only 25 (61%). Giardia lamblia was the most prevalent parasite (61%). Infections were more frequent among males (73%) and peaked during monsoon season (June–August), especially in children aged 11–15 years. Conclusion: The FEC method demonstrated higher sensitivity than direct wet mount. Routine use of FEC alongside wet mount microscopy can significantly improve the detection rate of intestinal parasites.