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Research Article Open access CC BY 4.0

Studies on Pancreatic Gene Expression in Diabetic Rats Treated with Moringa oleifera Leaf

Arvin Nwakulite, Emmanuel Ifeanyi Obeagu, H. U. Nwanjo, D. C. Nwosu, I. N. Nnatuanya, C. C. N. Vincent, Chukwudi Ofodile Amaechi, Ogochukwu, Mary-Theodora Barbara Ochiabu, Adaobi Maryann Ibekwe, Chukwuma J. Okafor, Getrude Uzoma Obeagu, Nkiruka Millicent Amadi

Journal of Pharmaceutical Research International · pp. 78–86 · Published 4 May 2021

10.9734/jpri/2021/v33i28A31512

Abstract

Moringa oleifera leaf have been used for treatment of diabetes, in this work we studied pancreatic gene expression. The gene expression was done by PCR machine. The plant was harvested from garden within Madonna University and was identified in the Department of Pharmacognosy of the University by Dr P. Osuagwu. Each of the extracts was analyzed for bioactive components using Gas Chromatography–Mass Spectrometry (GC-MS). Male wistar albino rats (n=40) six weeks old weighing 150-250g were purchased from the animal farm of Madonna University Elele. From this work we observed that, there was significant increase in the expression of ADIPOQ gene in the diabetic rats treated with 300mg/kg of M. oleifera leaf (1.52±0.13), when compared with that of the non-diabetic rats (1.00±0.02). From this study, it could be inferred that M. oleifera leaf powder contains polyphenols that inhibit carbohydrate metabolism, reduced blood glucose level, enhance expression ADIPOQ genes.

Pancreatic gene expression diabetic rats Moringa oleifera leaf

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