Demonstration of the Minimum Inhibitory Concentration (Mic) and Minimal Bactericidal Concentration(Mbc) of Both Moringa oleifera and Gongronema latifolium Extracts Mixture against Staphylococcus aureus, Salmonella typhi and Escherichia coli

Abstract

Background: Moringa oleifera and Gongronema latifolium have many nutritional values that contain bioactive components such as alkaloids, saponin, tannin, steroids and terpenoids, as  antimicrobials.            Objective: The general purpose of this study was to evaluate the minimum inhibitory concentration (MIC) and Minimal bactericidal concentration(MBC) of both Moringa oleifera and Gongronema latifolium  extracts against Staphylococcus aureus, Salmonella typhi and Escherichia coli. Method: This is laboratory experimental research involving post test only control group design using the Kirby Bauer dilution method. With treatment combination of Moringa oleifera and Gongronema latifolium extracts concentration ranging from 50-200 mg/ml and positive control (gentamycin). Result: Mininum bacterial concentration was found between 6.25 mg /ml – 12.5mg /ml for Moringa oleifera and Gongronema latifolium extracts while minimum inhibitory concentration of Moringa oleifera and Gongronema latifolium was found between 12.5 mg / ml – 25.0 mg/ml respectively. U-Mann whitney results showed that the value of p < 0.05 were significant. In this study, the Staphylococcus aureus showed the highest inhibition zone diameter compared to other isolates. Conclusion: The results from this work affirms the use of Moringa oleifera and Gongronema latifolium as antimicrobials and emplores the attention of pharmaceutical companies to exploit production of antibiotics for treatment of infections resulting from Salmonella typhi and Staphylococcus aureus isolates.