Genetic Diversity Study of Melon Native Landraces Using ISSR Markers in Kurdistan Region of Iraq
Aram Shahab Ahmed, Luqman Gharib Karim Barznjy, Sidiq Aziz Sidiq Kasnazany, Dara Omer Mohammed
Journal of Agriculture and Ecology Research International · pp. 213–217 · Published 23 Dec 2022
10.9734/jaeri/2022/v23i6512Abstract
Considering the ancient cultivation of various numbers of melons, it seems that there is a valuable and rich gene pool of melon in Kifri city of Kurdistan region of Iraq. In this research, the genetic diversity of 15 indigenous melons of Kifri and 7 non-native melons was analyzed using inter simple sequence repeat (ISSR) markers. A total of 6 primers were used to amplify parts of the genomic DNA of the samples. Genomic DNA was extracted using Salting out method, and polyacrylamide gel was used to separate DNA fragments. In this study, 65 bands were scored and 34 bands had polymorphisms. To evaluate the genetic similarity between the samples, cluster analysis was used using the Jaccard similarity coefficient with the UPGMA method. The average genetic distance between the samples using the Jaccard similarity coefficient was 0.74, and the average polymorphic information content (PIC) was 0.78. The primer (AC)8 G had the highest PIC (0.93). Cluster analysis indicated wide range of diversity across the genotypes used. These genotypes have high diversity which could be used in breeding programs.
Cited by 1
1 citation reported by external sources — individual citing-article records aren't available to list yet.
Related research
- Effect of Using Midazolam and Propofol in Low Doses for Intraoperative Nausea and Vomiting Prevention in Pregnant Women Underwent Cesarean Section under Regional Anesthesia — shares topic coverage
- Molecular Phylogenetic and Risk Factors of Klebsiella aerogenes in Diabetic Foot Ulcers: A First Study from Iraq — shares topic coverage
- Genetic Characterisation of Honey Bees (Apis mellifera) Populations from Kurdistan Region of Iraq via ISSR Markers — shares topic coverage
- Hydrochemical Evaluation of the Tigris River from Mosul to South of Baghdad Cities, Iraq — shares topic coverage
- Estimation of Environmental Chemical Pollution of Al-Baiji Oil Refinery in Iraq — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
1
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.