Free Radical Scavenging Activity and Phytochemical Investigation of Ethanol Leaf Extract of Dryopteris dilatata

Abstract

Bioactive constituents are the active ingredients of herbal product such as Dryopteris dilataa that possess therapeutic efficacies in the treatment and management of several diseases. Thus, we evaluated different concentration of the free radical scavenging activity and phytochemical constituents of ethanol and sub-extracts of Dryopteris dilatata (Dd) leaves such as butane, hexane and benzene. Phytochemicals analysis was determined on the ethanol extract of Dryopteris dilatata sub-extracts using standard principles, also free radical scavenging capacity of the ethanol extract for Ferric reducing antioxidant power (FRAP), hydroxyl radical scavenging assay (OH), superoxide radical scavenging assay (SSRA), 2,2--Diphenyl-1-Picrylhydrazyl assay (DPPH), 2, 2'-azino-bis 3-ethylbenzthiazoline-6-sulphoric acid scavenging activity (ABTS) and thiobarbituric acid (TBA) assay (TBA) was done using standard protocols. Results of the phytochemical analysis revealed flavonoids and cardiac glycosides in all extracts, terpenoid, phytosterol, alkaloid, tannins and saponin constituents were present in ethanol, butane and hexane extracts and absent in benzene extract, phenols is present in only hexane extract but absent in all other extracts, glycerols is present in only benzene extracts while phlobotannin and carbohydrate are absent in all extracts. The plant ethanol extract revealed statistically significant at P<0.05 capacity for (FRAP), (OH), (SSRA), (DPPH), (ABTS), (TA). The study therefore suggest that the plant extract could lead to producing therapeutic agent in the treatment and management of several disorders that are attributed to reactive species and oxidative stress.