Assessment of Plant Growth Promoting Traits of Bacterial Endophytes Resides in the Mulberry (Morus indica L.) through Laboratory Analysis

Abstract

Endophytes are the microorganisms living internal tissues of plants without causing any negative effect and they enhance plant growth by direct and indirect mechanisms. Mulberry is a crop mainly cultivated to rear silkworms for cocoon production further it leads to silk production. Th quality and quantity of mulberry leaves depending one of the important factor, fertilizer application. This study involved in the isolation and identification of growth promoting traits of bacterial endophytes present in the mulberry (Morus indica). The Plant Growth Promotion (PGP) assessments like IAA production, ammonia production, HCN production and siderophore production were carried out. Among obtained 30 isolates, all the isolates showed positive reaction for IAA test, 22 isolates for ammonia test, 12 isolates for siderophore production test and 2 isolates for HCN production tests showed positive reaction. Out of 30 isolates, 2 superior isolates were selected and identified as Enterobacter cloacae using 16s rRNA sequencing.