Molecular Detection of the Arr-2 Gene in Escherichia coli and Klebsiella pneumoniae Resistant to Rifampicin in Abidjan, Côte D'Ivoire
Victoire Gadou, Nathalie Kouadio Guessennd, Abalé A.Toty, Fernique Konan, Mohamed Baguy Ouattara, Mireille Dosso, Seydina M. Diène, Joseph Allico Djaman, Jean-Marc Rolain
Microbiology Research Journal International · pp. 1–8 · Published 11 Apr 2018
10.9734/MRJI/2018/40552Abstract
Aims: The aim of this study was to demonstrate the presence of rifampicin resistance arr-2 gene in certain enterobacteria of clinical origin from various biological products. Place and Duration of Study: National Reference Center for Antibiotics of Pasteur Institute of Côte d'Ivoire, and research unit on emerging tropical infectious diseases of Aix-Marseille University, between January to July 2017. Methodology: The strains were isolated from different biological samples and identified by biochemical tests and MALDI-TOF MS mass spectrometry. The Antibiotic susceptibility testing was performed by diffusion on Mueller- Hinton (MH) agar. Results: All strains were resistant to rifampicin (100%) and amoxicillin (100%), but sensitive to imipenem and colistin. Conventional PCR using specific primers detected the arr-2 gene in four strains, including 3 strains of Klebsiella pneumoniae and one strain of Escherichia coli. Molecular typing of the strains by MLST showed that the E. coli strain had ST 5 and the three strains of K. pneumoniae had ST 307, ST 309, ST 273 respectively. Conclusion: This article is the first report that highlights the presence of the arr-2 gene in enterobacteria in Côte d’Ivoire
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