Callus Induction, Regeneration and Establishment of Rice Plant from Mature Embryo

Abstract

Plant growth regulators were used to test callus induction and in vitro regeneration in six rice genotypes (RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97). Four different concentrations (1, 2, 3 and 4 mg/L) of 2,4-D for callus induction and three different concentrations (1,2 and 3 mg/L) of NAA with three doses (5,10 and 15 µ/L) of kinetin for callus regeneration were used to test the effect of plant growth regulators. This study found a high callus induction on MS medium enriched with 2 mg/L 2, 4-D. In cases of RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97, callus induction frequencies were 92.7%, 87.8%, 84.6%, 82.9%, 86.2% and 62.9%, respectively. In the regeneration, it was found that an MS medium enriched with 2 g/L Kinetin and 10 µm/L NAA has the ability to induce increased regeneration of different rice varieties (RM-AC-2 (72.4%), BRRI dhan89 (66.9%), BRRI dhan88 (62.5%), Nipponbare (63.3%), Koshihikari (48%) and Zenshan97 (39.6%). From the regenerated plants, one plant of the RM-AC-2 genotype availed to complete its life cycle and generated 32 effective tillers and yielded 89g. This rice plant is very promising for high yielding rice variety development program in Bangladesh. The improved callus development and regeneration ability of this genotype might be helpful for future rice variety development and genetic transformation program.