Effect of Different Plant Growth Regulators on In-vitro Regeneration in Varieties of Strawberry

Abstract

The present study was carried out in College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University (Imphal), Umiam, Meghalaya during 2017-18. The objective was to study the effect of growth regulators on in vitro regeneration in four varieties of strawberry viz., Camarosa, Chandler, Festival and Winter Dawn. Best sterilization results were obtained for explants sterilized using Tween 20, 0.1% Bavistin, 0.1% Ascorbic acid + 0.1% Streptomycin and 0.1% Mercuric chloride for 30, 45, 40 and 8 minutes respectively. Sixteen different concentrations and combinations of growth regulators were used out of which regeneration from runners was successfully observed in six combinations of growth regulators. MS media supplemented with 5 mg/l 6-Benzylaminopurine + 1 mg/l Indole-3-acetic Acid produced maximum percent survival of explants and maximum number of shoots while MS medium supplemented with 4 mg/l 6-Benzylaminopurine + 1 mg/l Indole-3-acetic Acid produced the highest shoot length. Rooting was observed in half strength MS media supplemented with 0.5 mg/l Indole-3-butyric acid. Rooted plantlets were transferred to soil where they showed 66.67% survival. MS medium supplemented with Kinetin were inferior in performance for shoot development to MS medium supplemented with various concentrations of 6- Benzylaminopurine