Organic Extracts of Pelargonium graveolens: Phenol Content, Anti-oxidant and Anti-bacterial Activities

Abstract

Aims: The aim of this study was determination of antioxidant activity and total phenolic content and antibacterial activity of Pelargonium graveolens organic extracts. Study Design: Different methods implemented for isolation and analysing of organic                               P. graveolens. Place and Duration of Study: Department of Genetics, Osmania University, Hyderabad, India, between March 2012 to December 2014. Methodology: The aerial parts were extracted by solvents (ethyl acetate and methanol) by two conventional methods to screen the ideal solvent for isolation of non-volatile extracts and calculate its phenolic content yield, level of anti-oxidant and anti-bacterial activity on bacterial strains. Results: Our results show the extraction of crude from ethyl acetate and methanol by two conventional methods. Both organic extracts were shown the presence of total phenolic content 53 mg/dry mass. Methanolic maceration extract were showing highest EC50 value (115.528 ug/mL) with low antioxidant level, whereas, Soxhlet process were exhibiting lowest EC50 value (47.666 ug/mL) with higher free radical activity with the standard ascorbic acid. The Methanolic extract were exhibiting best zone of inhibition on K. pneumonia (13 mm ) than ethyl acetate extract on E. coli (12 mm) was determined at concentration 20 ug/mL. The most significant antioxidant properties were observed in Soxhlet method by negative correlation with total phenolic content.  Conclusion: The results from this study have proven the information on the total phenolic, anti-oxidant level and anti-bacterial activity of organic extracts of P. graveolens. Further studies should be performed on other strains to strengthen the research. We conclude on the value of search non-volatile compounds for the medicinal value in the health care system.